A calibration method for the cross-coupling coefficients of linear accelerometers was researched in this paper. The calibration test was conducted on a precision centrifuge. The calculation of radius and the procedure of iterative regression method were analyzed in this paper. According to the principle of measurement, the source of alignment error were analyzed in the installation condition of both the crossed-axis and the transverse axis. The calibration accuracy was ensured by the calculation of radius,the definition of alignment error for nominal installation angle and the research of influence on the second-order coefficients by alignment error. The modeling method was established and the uncertainty of calibration result was analyzed.