Wireless pressure acquisition instruments are widely used for calibrating pressure sterilization and other pressure vessel. The application temperature of wireless pressure acquisition instruments is different from other pressure instruments. A kind of calibration device was designed for wireless pressure acquisition instruments. The device adopts three-section temperature control and automatic pressure regulation, and can be used for automatic calibration of wireless pressure acquisition instruments. The experimental results show that the uncertainty of the device can meet the requirement of the wireless pressure acquisition calibration.