Frequency transmission based on optical fibers plays an important role in the construction and application of time?frequency synchronization systems. In order to quantify the effect of delay fluctuations and dispersive residuals introduced by temperature and wavelength asymmetries on the stability of the stable radio frequency transmission system, a simple frequency transmission system and a 3 000 km fiber link are constructed using optical simulation software for simulation and analysis of 10 GHz RF signal transmission. Through analysis, it is found that the temperature asymmetry introduced in the link has a small effect on system frequency stability, while the wavelength asymmetry has a larger effect. The stability of the system deteriorates by more than 10^-13/ s as the difference between the positive and negative wavelength increases. This study has a practical guiding effect on the construction and optimization of the long distance optical fiber frequency transmission system in the actual environment.