Aiming at the problem of double closed-loop tracking control of dew point temperature in high-precision quartz resonant dew point meter, a temperature control system of resonant dew point meter based on double semiconductor cooler was proposed. The system uses STM32 as the main control chip and uses PID control algorithm to drive and follow the temperature of two semiconductor coolers through DAC. The temperature control system is tested in the temperature range of -15~15 ℃. The results show that the linear cooling rate error of the main temperature control system is no more than 10%, and the continuous linear temperature control precision is high. It can provide an effective temperature control solution for the high-precision quartz resonant dew point meter based on double cooling surfaces to realize double closed-loop dew point tracking measurement.