Aiming at the calibration problem of single beam pulse laser velocimeter in laboratory environment, this paper presents a design and implementation method of moving target simulator, which is based on the generation principle of high-precision pulse sequence with time delay. In this paper, a specific delay pulse sequence is designed. Through software programming, the data timing generator is controlled as the hardware circuit generating equipment. The storage and distribution of the delay pulse sequence and the circuit generation are completed. The universal moving target simulator with any standard speed is realized, and the laser velocimeter verification device is constructed. The experimental results show that the device has good repeatability.