In the process of gyro theodolite calibration, the center of gyro theodolite and observation center of astronomical orientation should be aligned. Its alignment accuracy directly affects the north-seeking precision. In this paper, a double target accurate alignment method based on two parallel collimators is introduced. This method can realize the high precision alignment between the center of gyro theodolite and the observation center of astronomical orientation. At the same time, in order to match the use of this method, a gyro theodolite position adjustment mechanism is introduced. The mechanism can realize horizontal two-dimensional displacement adjustment and high and low altitude adjustment of gyro theodolite. By adjusting the altitude, the center of the gyro theodolite and the observatory center of astronomical orientation are aligned with high precision, thus the measurement accuracy is improved and the calibration requirements of the different types of Gyro theodolite can be met.