This paper briefly describes the function, installation and how to use the software on Windows 95 of PC - DIO-96 board, which is used for data acquisition, and supplied by National Instruments. It gives the program flow that run on Windows for accomplishing PC- DIO- 96's line,port and group application. It presents the details of application for the project of " Establish the large dimension etalon up to 60m", which include environment data acquisition and controlling motor.