The traditional method for calibrating strobe using simulation testers requires visual judgment by operators, resulting in poor repeatability of test results and large uncertainty in the evaluation results. A laboratory calibration method for helicopter rotor cone strobe, a specialized aviation testing equipment, was studied. This method uses the pulse frequency method to establish a calibration device in the form of photoelectric conversion to calibrate technical parameters such as the flashing frequency of the strobe. The experimental results show that while the accuracy has been significantly improved, and the uncertainty is smaller. Compared with the traditional method, this method is simple and fast to operate, and universal standard equipment can meet the calibration requirements. The traceability of measurement values is more reliable and easier, and it also meets the requirements of metrology regulations, and has certain popularization and application value.