In order to study the effect of the variation of the received signal amplitude of the acoustic velocity method on the temperature field reconstruction, a thermal calibration wind tunnel simulation test was conducted based on the acoustic thermometry method to successfully simulate the temperature field reconstruction at the exit of an aero-engine combustion chamber. Firstly, features are extracted from the received signal data and a feature matrix is established to feedback the change of signal amplitude. Then, based on the collected signal data, the temperature field reconstruction is carried out by the least squares method. Finally, the effect of the change of signal amplitude on the temperature field reconstruction by the acoustic velocity method is analyzed by comparing the actual effects of temperature field reconstruction under different feature matrices. Through experimental verification, it can be seen that: the larger the received signal amplitude is, the larger the root mean square error of the reconstructed temperature field is, when the amplitude is 40% greater than the theoretical value, the root mean square error is 14.38% greater than the theoretical value; the smaller the received signal amplitude is, the larger the maximum relative error of the reconstructed temperature field is, when the amplitude is 40% less than the theoretical value, the maximum relative error is 44.3762 K greater than the theoretical value.The research in this paper has an important technical reference value to promote the development of acoustic temperature measurement technology in the field of aero-engine combustion chamber outlet temperature field testing.